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1994-10-25
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Document 3053
DOCN M94A3053
TI Precision and stability of CD4, 8 and 3 values in HIV+ and
HIV-populations in Shirati Tanzania as evaluated by FACSCount.
DT 9412
AU Strauss K; Brubaker G; Kumbi D; Shao J; Krenkler W; Blattner W; Levin A;
Becton Dickinson, Europe, Belgium.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):164 (abstract no. PB0084). Unique
Identifier : AIDSLINE ICA10/94369522
AB The performance characteristics of a dedicated CD4 counting system,
FACSCount, were evaluated in populations from Shirati, Tanzania.
Precision assessments involved 4 replicate stainings and analyses per
subject. The pooled CV's of the parameters evaluated (CD4,8,3 and 4:8
ratio) are presented below: TABULAR DATA, SEE ABSTRACT VOLUME. Stability
assessments involved 4 replicate analyses per subject, at time points 0,
24, 48 and 72 hours, of both unstained aliquots and aliquots stained at
0 hour. Aged blood testing revealed no significant differences between
any time points. ANOVA aged stain testing revealed the following
differences (p < 0.05): TABULAR DATA, SEE ABSTRACT VOLUME. CONCLUSION:
FACSCount provides precise and stable stained and unstained results in
the large majority of HIV- and HIV+ Tanzanian subjects tested.
DE Antigens, CD3/*BLOOD Antigens, CD4/*BLOOD Antigens, CD8/*BLOOD
CD4-CD8 Ratio *Developing Countries Human HIV
Seronegativity/*IMMUNOLOGY HIV
Seropositivity/CLASSIFICATION/DIAGNOSIS/*IMMUNOLOGY Leukocyte Count
Reference Values Reproducibility of Results T-Lymphocyte
Subsets/*IMMUNOLOGY Tanzania T4 Lymphocytes/IMMUNOLOGY MEETING
ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).